| Competition: | Luxembourg National Division |
| Season: | 1999–2000 |
| Winners: | F91 Dudelange (1st title) |
| Continentalcup1: | Champions League |
| Continentalcup1 Qualifiers: | F91 Dudelange |
| Continentalcup2: | UEFA Cup |
| Continentalcup2 Qualifiers: | Grevenmacher Jeunesse Esch (via cup) |
| Continentalcup3: | Intertoto Cup |
| Continentalcup3 Qualifiers: | Hobscheid |
| Relegated: | Aris Bonnevoie Schifflange 95 |
| Prevseason: | 1998–99 |
| Nextseason: | 2000–01 |
The 1999–2000 Luxembourg National Division was the 86th season of top level association football in Luxembourg.
It was performed in 12 teams, and F91 Dudelange won the championship after a play off phase.