CBFB explained
Core-binding factor subunit beta is a protein that in humans is encoded by the CBFB gene.[1]
The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by the alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene.[2]
Mutations in CBFB are implicated in cases of breast cancer.[3]
Core binding factor acute myeloid leukaemia is a cancer related to genetic changes in the CBF gene. It is most commonly caused by an inversion of particular region of chromosome 16; however it can also be caused by translocation between copies of chromosome 16. The rearrangements cause formation of CBF but with impaired function. This prevents proper differentiation of blood cells, leading to the formation of Myeloblast.[4]
Further reading
- Hart SM, Foroni L . Core binding factor genes and human leukemia. . Haematologica . 87 . 12 . 1307–23 . 2003 . 12495904 .
- Bae SC, Takahashi E, Zhang YW . Cloning, mapping and expression of PEBP2 alpha C, a third gene encoding the mammalian Runt domain. . Gene . 159 . 2 . 245–8 . 1995 . 7622058 . 10.1016/0378-1119(95)00060-J . etal.
- Bonaldo MF, Lennon G, Soares MB . Normalization and subtraction: two approaches to facilitate gene discovery. . Genome Res. . 6 . 9 . 791–806 . 1997 . 8889548 . 10.1101/gr.6.9.791 . free .
- Hillier LD, Lennon G, Becker M . Generation and analysis of 280,000 human expressed sequence tags. . Genome Res. . 6 . 9 . 807–28 . 1997 . 8889549 . 10.1101/gr.6.9.807 . etal. free .
- Hajra A. Collins FS.
- Chiba N, Watanabe T, Nomura S . Differentiation dependent expression and distinct subcellular localization of the protooncogene product, PEBP2beta/CBFbeta, in muscle development. . Oncogene . 14 . 21 . 2543–52 . 1997 . 9191054 . 10.1038/sj.onc.1201109 . etal. free .
- Tanaka Y, Watanabe T, Chiba N . The protooncogene product, PEBP2beta/CBFbeta, is mainly located in the cytoplasm and has an affinity with cytoskeletal structures. . Oncogene . 15 . 6 . 677–83 . 1997 . 9264408 . 10.1038/sj.onc.1201235 . 7192875 . etal.
- Kitabayashi I, Ida K, Morohoshi F . The AML1-MTG8 leukemic fusion protein forms a complex with a novel member of the MTG8(ETO/CDR) family, MTGR1. . Mol. Cell. Biol. . 18 . 2 . 846–58 . 1998 . 9447981 . 10.1128/MCB.18.2.846. 108796 . etal.
- Levanon D, Goldstein RE, Bernstein Y . Transcriptional repression by AML1 and LEF-1 is mediated by the TLE/Groucho corepressors. . Proc. Natl. Acad. Sci. U.S.A. . 95 . 20 . 11590–5 . 1998 . 9751710 . 10.1073/pnas.95.20.11590 . 21685 . 1998PNAS...9511590L . etal. free .
- Kogan SC, Lagasse E, Atwater S . The PEBP2betaMYH11 fusion created by Inv(16)(p13;q22) in myeloid leukemia impairs neutrophil maturation and contributes to granulocytic dysplasia. . Proc. Natl. Acad. Sci. U.S.A. . 95 . 20 . 11863–8 . 1998 . 9751756 . 10.1073/pnas.95.20.11863 . 21731 . 1998PNAS...9511863K . etal. free .
- Goger M, Gupta V, Kim WY . Molecular insights into PEBP2/CBF beta-SMMHC associated acute leukemia revealed from the structure of PEBP2/CBF beta. . Nat. Struct. Biol. . 6 . 7 . 620–3 . 1999 . 10404215 . 10.1038/10664 . 13114284 . etal.
- Lutterbach B, Hou Y, Durst KL, Hiebert SW . The inv(16) encodes an acute myeloid leukemia 1 transcriptional corepressor. . Proc. Natl. Acad. Sci. U.S.A. . 96 . 22 . 12822–7 . 1999 . 10536006 . 10.1073/pnas.96.22.12822 . 23113 . 1999PNAS...9612822L . free .
- Warren AJ, Bravo J, Williams RL, Rabbitts TH. Roger L. Williams . Structural basis for the heterodimeric interaction between the acute leukaemia-associated transcription factors AML1 and CBFbeta. . . 19 . 12 . 3004–15 . 2000 . 10856244 . 10.1093/emboj/19.12.3004 . 203359 .
- Kanto S, Chiba N, Tanaka Y . The PEBP2beta/CBF beta-SMMHC chimeric protein is localized both in the cell membrane and nuclear subfractions of leukemic cells carrying chromosomal inversion 16. . Leukemia . 14 . 7 . 1253–9 . 2000 . 10914550 . 10.1038/sj.leu.2401821 . 22471555 . etal.
- O'Reilly J, Chipper L, Springall F, Herrmann R . A unique structural abnormality of chromosome 16 resulting in a CBF beta-MYH11 fusion transcript in a patient with acute myeloid leukemia, FAB M4. . Cancer Genet. Cytogenet. . 121 . 1 . 52–5 . 2000 . 10958941 . 10.1016/S0165-4608(00)00235-1 .
- Bravo J, Li Z, Speck NA, Warren AJ . The leukemia-associated AML1 (Runx1)--CBF beta complex functions as a DNA-induced molecular clamp. . Nat. Struct. Biol. . 8 . 4 . 371–8 . 2001 . 11276260 . 10.1038/86264 . 23570097 .
- Bäckström S, Wolf-Watz M, Grundström C . The RUNX1 Runt domain at 1.25A resolution: a structural switch and specifically bound chloride ions modulate DNA binding. . J. Mol. Biol. . 322 . 2 . 259–72 . 2002 . 12217689 . 10.1016/S0022-2836(02)00702-7 . etal.
Notes and References
- Liu P, Seidel N, Bodine D, Speck N, Tarle S, Collins FS . Acute myeloid leukemia with Inv (16) produces a chimeric transcription factor with a myosin heavy chain tail . Cold Spring Harb Symp Quant Biol . 59 . 547–53 . Nov 1995 . 7587111 . 10.1101/sqb.1994.059.01.061.
- Web site: Entrez Gene: CBFB core-binding factor, beta subunit.
- The Cancer Genome Atlas Network . 2012 . Comprehensive molecular portraits of human breast tumours . Nature . 490. 7418. 61–70. Nature Publishing Group . 10.1038/nature11412 . 23000897 . 3465532. 2012Natur.490...61T .
- "CBFB Gene." Genetics Home Reference. US National Library of Medicine, 10 Sept. 2015. Web. 27 Sept. 2015.