RBX1 explained
RING-box protein 1 is a protein that in humans is encoded by the RBX1 gene.[1] [2]
Function
This gene encodes an evolutionarily conserved protein that interacts with cullins. The protein plays a unique role in the ubiquitination reaction by heterodimerizing with cullin-1 to catalyze ubiquitin polymerization. It also may be involved in the regulation of protein turn-over.[3]
Interactions
RBX1 has been shown to interact with:
Further reading
- Skowyra D, Koepp DM, Kamura T, Conrad MN, Conaway RC, Conaway JW, Elledge SJ, Harper JW . Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1 . Science . 284 . 5414 . 662–5 . Apr 1999 . 10213692 . 10.1126/science.284.5414.662 . 1999Sci...284..662S .
- Tan P, Fuchs SY, Chen A, Wu K, Gomez C, Ronai Z, Pan ZQ . Recruitment of a ROC1-CUL1 ubiquitin ligase by Skp1 and HOS to catalyze the ubiquitination of I kappa B alpha . Molecular Cell . 3 . 4 . 527–33 . Apr 1999 . 10230406 . 10.1016/S1097-2765(00)80481-5 . free .
- Winston JT, Koepp DM, Zhu C, Elledge SJ, Harper JW . A family of mammalian F-box proteins . Current Biology . 9 . 20 . 1180–2 . Oct 1999 . 10531037 . 10.1016/S0960-9822(00)80021-4 . free . 1999CBio....9.1180W .
- Kamura T, Conrad MN, Yan Q, Conaway RC, Conaway JW . The Rbx1 subunit of SCF and VHL E3 ubiquitin ligase activates Rub1 modification of cullins Cdc53 and Cul2 . Genes & Development . 13 . 22 . 2928–33 . Nov 1999 . 10579999 . 317157 . 10.1101/gad.13.22.2928 .
- Dunham I, Shimizu N, Roe BA, Chissoe S, Hunt AR, Collins JE, Bruskiewich R, Beare DM, Clamp M, Smink LJ, Ainscough R, Almeida JP, Babbage A, Bagguley C, Bailey J, Barlow K, Bates KN, Beasley O, Bird CP, Blakey S, Bridgeman AM, Buck D, Burgess J, Burrill WD, O'Brien KP . The DNA sequence of human chromosome 22 . Nature . 402 . 6761 . 489–95 . Dec 1999 . 10591208 . 10.1038/990031 . 1999Natur.402..489D . free .
- Seddiqi N, Charbonnier F, Goudou D, Belkadi L, Rieger F, Alliel PM . Genomic organization and expression of the ubiquitin-proteasome complex-associated protein Rbx1/ROC1/Hrt1 . Cellular and Molecular Biology . 45 . 8 . 1131–7 . Dec 1999 . 10643962 .
- Read MA, Brownell JE, Gladysheva TB, Hottelet M, Parent LA, Coggins MB, Pierce JW, Podust VN, Luo RS, Chau V, Palombella VJ . Nedd8 modification of cul-1 activates SCF(beta(TrCP))-dependent ubiquitination of IkappaBalpha . Molecular and Cellular Biology . 20 . 7 . 2326–33 . Apr 2000 . 10713156 . 85397 . 10.1128/MCB.20.7.2326-2333.2000 .
- Furukawa M, Zhang Y, McCarville J, Ohta T, Xiong Y . The CUL1 C-terminal sequence and ROC1 are required for efficient nuclear accumulation, NEDD8 modification, and ubiquitin ligase activity of CUL1 . Molecular and Cellular Biology . 20 . 21 . 8185–97 . Nov 2000 . 11027288 . 86428 . 10.1128/MCB.20.21.8185-8197.2000 .
- Maeda I, Ohta T, Koizumi H, Fukuda M . In vitro ubiquitination of cyclin D1 by ROC1-CUL1 and ROC1-CUL3 . FEBS Letters . 494 . 3 . 181–5 . Apr 2001 . 11311237 . 10.1016/S0014-5793(01)02343-2 . free .
- Lyapina S, Cope G, Shevchenko A, Serino G, Tsuge T, Zhou C, Wolf DA, Wei N, Shevchenko A, Deshaies RJ . Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalosome . Science . 292 . 5520 . 1382–5 . May 2001 . 11337588 . 10.1126/science.1059780 . 2001Sci...292.1382L . 14224920 .
- Fukuchi M, Imamura T, Chiba T, Ebisawa T, Kawabata M, Tanaka K, Miyazono K . Ligand-dependent degradation of Smad3 by a ubiquitin ligase complex of ROC1 and associated proteins . Molecular Biology of the Cell . 12 . 5 . 1431–43 . May 2001 . 11359933 . 34595 . 10.1091/mbc.12.5.1431 .
- Kamura T, Burian D, Yan Q, Schmidt SL, Lane WS, Querido E, Branton PE, Shilatifard A, Conaway RC, Conaway JW . Muf1, a novel Elongin BC-interacting leucine-rich repeat protein that can assemble with Cul5 and Rbx1 to reconstitute a ubiquitin ligase . The Journal of Biological Chemistry . 276 . 32 . 29748–53 . Aug 2001 . 11384984 . 10.1074/jbc.M103093200 . free .
- Wu K, Chen A, Tan P, Pan ZQ . The Nedd8-conjugated ROC1-CUL1 core ubiquitin ligase utilizes Nedd8 charged surface residues for efficient polyubiquitin chain assembly catalyzed by Cdc34 . The Journal of Biological Chemistry . 277 . 1 . 516–27 . Jan 2002 . 11675391 . 10.1074/jbc.M108008200 . free .
- Gomes MD, Lecker SH, Jagoe RT, Navon A, Goldberg AL . Atrogin-1, a muscle-specific F-box protein highly expressed during muscle atrophy . Proceedings of the National Academy of Sciences of the United States of America . 98 . 25 . 14440–5 . Dec 2001 . 11717410 . 64700 . 10.1073/pnas.251541198 . free . 2001PNAS...9814440G .
- Kim J, Kim JH, Lee SH, Kim DH, Kang HY, Bae SH, Pan ZQ, Seo YS . The novel human DNA helicase hFBH1 is an F-box protein . The Journal of Biological Chemistry . 277 . 27 . 24530–7 . Jul 2002 . 11956208 . 10.1074/jbc.M201612200 . free .
- Zheng N, Schulman BA, Song L, Miller JJ, Jeffrey PD, Wang P, Chu C, Koepp DM, Elledge SJ, Pagano M, Conaway RC, Conaway JW, Harper JW, Pavletich NP . Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex . Nature . 416 . 6882 . 703–9 . Apr 2002 . 11961546 . 10.1038/416703a . 2002Natur.416..703Z . 4423882 .
- Yoshida Y, Chiba T, Tokunaga F, Kawasaki H, Iwai K, Suzuki T, Ito Y, Matsuoka K, Yoshida M, Tanaka K, Tai T . E3 ubiquitin ligase that recognizes sugar chains . Nature . 418 . 6896 . 438–42 . Jul 2002 . 12140560 . 10.1038/nature00890 . 2002Natur.418..438Y . 4424996 .
- Brower CS, Sato S, Tomomori-Sato C, Kamura T, Pause A, Stearman R, Klausner RD, Malik S, Lane WS, Sorokina I, Roeder RG, Conaway JW, Conaway RC . Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase . Proceedings of the National Academy of Sciences of the United States of America . 99 . 16 . 10353–8 . Aug 2002 . 12149480 . 124918 . 10.1073/pnas.162424199 . free . 2002PNAS...9910353B .
Notes and References
- Kamura T, Koepp DM, Conrad MN, Skowyra D, Moreland RJ, Iliopoulos O, Lane WS, Kaelin WG, Elledge SJ, Conaway RC, Harper JW, Conaway JW . Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase . Science . 284 . 5414 . 657–61 . Apr 1999 . 10213691 . 10.1126/science.284.5414.657 . 1999Sci...284..657K .
- Ohta T, Michel JJ, Schottelius AJ, Xiong Y . ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity . Molecular Cell . 3 . 4 . 535–41 . Apr 1999 . 10230407 . 10.1016/S1097-2765(00)80482-7 . 19371828 . free .
- Web site: Entrez Gene: RBX1 ring-box 1.
- Min KW, Hwang JW, Lee JS, Park Y, Tamura TA, Yoon JB . TIP120A associates with cullins and modulates ubiquitin ligase activity . The Journal of Biological Chemistry . 278 . 18 . 15905–10 . May 2003 . 12609982 . 10.1074/jbc.M213070200 . free .
- Kim AY, Bommeljé CC, Lee BE, Yonekawa Y, Choi L, Morris LG, Huang G, Kaufman A, Ryan RJ, Hao B, Ramanathan Y, Singh B . SCCRO (DCUN1D1) is an essential component of the E3 complex for neddylation . The Journal of Biological Chemistry . 283 . 48 . 33211–20 . Nov 2008 . 18826954 . 2586271 . 10.1074/jbc.M804440200 . free .
- Kim J, Kim JH, Lee SH, Kim DH, Kang HY, Bae SH, Pan ZQ, Seo YS . The novel human DNA helicase hFBH1 is an F-box protein . The Journal of Biological Chemistry . 277 . 27 . 24530–7 . Jul 2002 . 11956208 . 10.1074/jbc.M201612200 . free .
- Zheng N, Schulman BA, Song L, Miller JJ, Jeffrey PD, Wang P, Chu C, Koepp DM, Elledge SJ, Pagano M, Conaway RC, Conaway JW, Harper JW, Pavletich NP . Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex . Nature . 416 . 6882 . 703–9 . Apr 2002 . 11961546 . 10.1038/416703a . 2002Natur.416..703Z . 4423882 .
- Dias DC, Dolios G, Wang R, Pan ZQ . CUL7: A DOC domain-containing cullin selectively binds Skp1.Fbx29 to form an SCF-like complex . Proceedings of the National Academy of Sciences of the United States of America . 99 . 26 . 16601–6 . Dec 2002 . 12481031 . 139190 . 10.1073/pnas.252646399 . free .
- Guerrero-Santoro J, Kapetanaki MG, Hsieh CL, Gorbachinsky I, Levine AS, Rapić-Otrin V . The cullin 4B-based UV-damaged DNA-binding protein ligase binds to UV-damaged chromatin and ubiquitinates histone H2A . Cancer Research . 68 . 13 . 5014–22 . Jul 2008 . 18593899 . 10.1158/0008-5472.CAN-07-6162 . free .
- Duda DM, Borg LA, Scott DC, Hunt HW, Hammel M, Schulman BA . Structural insights into NEDD8 activation of cullin-RING ligases: conformational control of conjugation . Cell . 134 . 6 . 995–1006 . Sep 2008 . 18805092 . 2628631 . 10.1016/j.cell.2008.07.022 .
- Panasyuk G, Nemazanyy I, Filonenko V, Gout I . Ribosomal protein S6 kinase 1 interacts with and is ubiquitinated by ubiquitin ligase ROC1 . Biochemical and Biophysical Research Communications . 369 . 2 . 339–43 . May 2008 . 18279656 . 10.1016/j.bbrc.2008.02.016 .